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The records of 80 patients who underwent surgery for aortic root replacement for infective endocarditis from January 1980 to December 1988 were reviewed. All cases were reevaluated from the time of diagnosis and surgery to the present. The incidence of concomitant aortic valve repair during aortic valve replacement increased from 9% in 1980 to 21% in 1988. Recent onset of infection for those older than 50 years old and presentation with severe aortic regurgitation or renal failure were statistically significant predictors of operative mortality. Three patients underwent delayed aortic valve replacement 6 days to 4 years after aortic root replacement, none died of infection. Early aortic valve replacement appears to be the treatment of choice in cases of active infection because it minimizes the need for concomitant aortic valve repair.Signal transduction in Escherichia coli: down regulation of diacylglycerol acyltransferase by phosphatidic acid.
A strain of Escherichia coli defective in diacylglycerol acyltransferase (Dga) activity was used to assess the physiological importance of Dga-catalyzed conversion of phosphatidic acid (PA) to diacylglycerol (DAG). Phenotypic analysis of this mutant suggested that Dga is important for maintaining normal cellular levels of DAG and triacylglycerol in cells growing on a variety of carbon sources, including palmitate, suggesting that Dga is the physiologically relevant isoform of diacylglycerol acyltransferase in E. coli. In cells in which PA was exogenously added, Dga activity was induced several-fold, whereas the cellular level of DAG decreased by more than 70%. These changes were accompanied by the appearance of an acid-soluble phosphatidylinositol-specific phospholipase C (PI-PLC) activity. These results suggested that an exogenous supply of PA is converted to DAG in an inactivated form. In cells grown in a PA-
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