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The mechanism of action of taxol, an antitumor agent, has been examined using cultured human fibroblast cells. Taxol caused the tyrosine phosphorylation of several proteins of 160, 94, and 83 kDa. Among them, the 94-kDa protein was identified as c-Src by its antibody-specific phosphorylation. The phosphorylation of c-Src and other cellular proteins induced by taxol was dose- and time-dependent. The tyrosine phosphorylation of these proteins was inhibited by both antibodies against c-Src and by PP2, a Src-family kinase inhibitor. The c-Src phosphorylation was localized to the plasma membrane and nucleus. The tyrosine phosphorylation of these proteins induced by taxol was completely abrogated by pretreatment with the antimitotic agent, nocodazole, which interferes with microtubule assembly. Inhibition of protein tyrosine kinase by tyrphostin AG1478 or genistein had the effect of protecting human fibroblast cells from taxol-induced cytotoxicity. These results suggest that taxol induces the tyrosine phosphorylation of c-Src by activating Src-family kinases in the plasma membrane and then causes the collapse of the microtubule network. This results in the activation of caspases, leading to apoptosis.The microRNA miR-137 was specifically induced by glucocorticoid in cardiac muscle cells and attenuated glucocorticoid induced apoptosis by targeting Bcl-2.
The aim of this study was to eluc
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Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
Indian Freedom Struggle By Venkatesan Pdf 18lkjhl.
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