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We have begun to examine the functional role of metabolic processes in basophils by examining the influence of metabolites on the in vitro activation of these cells with anti-IgE. We have found that nonmetabolizable glucose analogs, 6-deoxyglucose and 2-deoxyglucose, inhibit the ability of tumor mast cells to release histamine from preloaded cells. These data indicate a requirement for metabolic processes. It is likely that a metabolite of glycolysis, such as citrate, is involved in the activation of basophils, because the inhibitory effect of 6-deoxyglucose is mimicked by the pyruvate analog 2-deoxyglucose. 6-Deoxyglucose also inhibited the release of the calcium-independent phospholipase A2 from PMHS A2B5 tumor mast cells. In contrast, other metabolites, especially ATP, did not mimic the effects of 2-deoxyglucose, indicating that this glycolytic intermediate is not the second messenger involved in the activation of these cells. 2-Deoxyglucose did not, however, inhibit the activation of tumor mast cells by secretagogues other than anti-IgE (i.e. compound 48/80, phorbol ester, and N-formyl-methionyl-leucyl-phenylalanine). The reason for this difference is not known.Reading Time: 5 minutes
It is merely an assumption, but one that nobody can discount, that we are going to have a number of governments close down in the not too distant future. Whilst this is a real risk for all Australians, it is a risk that is greater for regional and remote communities.
Recently, the 18,000 Australian Government employees working for the Commonwealth in the Kimberley have been without a government contract since June 30. The loss of this huge workforce in the region is estimated to have been worth between $300 million and $600 million.
This has been the state of affairs for several months now. The Department of Employment refused to provide services to the Kimberley region to assist with the re-opening of the Kimberley economy when the coronavirus pandemic came to Western Australia last month.
This decision led
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